Multiple active conformers of mouse ornithine decarboxylase
نویسندگان
چکیده
منابع مشابه
Strain polymorphism and tentative mapping of mouse ornithine decarboxylase.
SOURCE/DESCRIPTION The plasmld p0D45~contains a 1.6 kb of a omithine decarboxyiase cDNA . POLYMORPHISM Constant Eco RI bands vere found at 3.6, 4.4, 5.5, 6.9, 8.5, 11.5, 13.5 and 19 kb. Variant bands vere found at 4.7 and 6.2 kb. FREQUENCY The 4.7 band is present in BALB/c, not in C57BL/6, DBA/2 and C3Hf, and segregate in CxB recombinant inbred (RI) strains. The 6.2 kb band is present in DBA/2 ...
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Ornithine decarboxylase, the first enzyme of the polyamine biosynthetic pathway, is induced by androgens in the mouse kidney. We have isolated from a kidney cDNA clone bank two plasmids, pODC1440 and pODC934 , that contain different cDNA inserts corresponding to ornithine decarboxylase mRNA. Identification was based upon the ability of plasmid-specific mRNAs to encode a 53,000-dalton polypeptid...
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We used mRNA from a mutant S49 mouse lymphoma cell line that produces ornithine decarboxylase (OrnDCase) as its major protein product to synthesize and clone cDNA. Plasmids containing OrnDCase cDNA were identified by hybrid selection of OrnDCase mRNA and in vitro translation. The two of these with the largest inserts together span 2.05 kilobases of cDNA. Southern blot analysis of DNA from wild-...
متن کاملMultiple regulation of ornithine decarboxylase in enzyme-overproducing cells.
We have isolated from mouse FM3A cells a variant cell line, termed EXOD-1, that overproduces ornithine decarboxylase (ODC). The cells were resistant to alpha-difluoromethylornithine, an irreversible inhibitor of the enzyme, and produced the enzyme protein to the extent of approx. 3-6% of total cytosolic protein. The rate of ODC synthesis in this cell line accounted for 25-50% of the rate of tot...
متن کاملMouse ornithine decarboxylase gene: cloning, structure, and expression.
We used molecular cloning to isolate a functional gene for mouse ornithine decarboxylase (OrnDCase; L-ornithine carboxy-lyase, EC 4.1.1.17) from a cell line in which that gene had been selectively amplified. The position of the 5' terminus of the mRNA was identified, and the coding sequence was shown to be preceded by a 312- or 313-nucleotide (nt) untranslated leader. The latter is highly G + C...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1993
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2930289